Analysis of a Long Covid Microbiome Sample

The reader describes their situation below:


I am living with LongCOVID following infection in March / April 2020. I contracted COVID-19 in the workplace, employed as a pharmacist at an NHS hospital in South Wales, U.K.

I  shared my story with WalesOnline at the latter stages of 2020 due to the lack of awareness around LongCOVID, and I share with you below for your interest.

📺 ‘Super-fit pharmacist who has ‘long Covid’ now left breathless by short walks’

📺 ‘30-year-old fitness fanatic with long Covid details his horrendous list of symptoms’

Unfortunately, I am still troubled by GI symptoms and despite improving over the past few months, I’m still having difficulty with bowel urgency / diarrhoea and mild abdominal pain. I lost 10kg in 10 weeks between July – Sept. 2020 (72kg at my lowest); thankfully this has recovered and I have gained weight, albeit chubbiness, weighing 88kg last week. I was diagnosed at the start of 2021 with ‘post-viral IBS’ and ‘leaky gut syndrome’, but GI clinicians are at a loss of how to proceed with my symptoms, hence my purchase of the BiomeSight kit. I have tried numerous diets (FODMAP, dairy-, gluten-free), again, to no avail.

I approach my 20th month since first being infected and I am still quite a distance from where I was pre-COVID doing all I possibly can to recover, so I would be extremely grateful for your insight, not only to help myself, but others in a similar situation.

Approach #1

As we have two microbiome profiles for COVID from the US Nation Library of Medicine, I will apply each one using 6%ile filter (values in the top or bottom 6%ile) to get a feel for the ground work. Then I will apply the ME/CFS for a third one (because of the similarity of Long COVID and ME/CFS).

For those not familiar with selecting

We end up with a short lists of bacteria (the titles links to the bacteria and studies reporting these shifts)

Active COVID 230 suggestions
Long COVID 280 suggestions
Chronic Fatigue Syndrome 230 suggestions

Almost everything is too low. Rather than examining suggestions from each of them, I will go directly to the consensus report. We hit a surprising 108 items on the safest take (items that will not shift any of the above in the wrong direction). Most are recommended in each case (Take Count = 3)

Safest Take

A few quick notes: Apples are very rich in pectin (some studies used apples and other pectin — I always try to keep data as reported and not do ‘well it’s just like…’ simplification). Similar with inulin and chicory.

The Safer text (some pro and some con) list was short and a bit of a mixed bag. With 108 items on safest, I would tend to ignore these. No need to include them.

On the avoid list we have “magnesium deficient diet” — which usually translates to magnesium rich or supplements.

I attach the complete list below of 304 different items.

Approach #2

This person is a pharmacist and thus looking at off-label drugs may be interesting for him to review. There are no accepted drugs for Long COVID, however, for ME/CFS often the top off-label drugs have often been used (with good results) by ME/CFS specialist (often at risk of professional censure). I have also added in CFS/ME with IBS (only Bacteroides Low was a match), and IBS to the consensus report.

The Criteria Selection being tried
Bacteria selected from IBS

The number of drugs that could influence these bacteria (good or bad) was almost 1300. I included some non prescription items to serve as a reference point (i.e. do drugs do better than some alternatives). In the small list of antibiotics at the top, I see several of the works for ME/CFS antibiotics — especially, those used by Cecile Jadin, MD: Tetracyclines, macrolides. Jadin does antibiotic rotation: 10 days on and 20 days off, then change to the next antibiotic. I have seen a few PubMed studies finding rotation was superior.

I noticed that several antibiotics often used for ME/CFS and IBS was on the avoid list: rifaximin (antibiotic)s, azithromycin,(antibiotic)s

The full list is attached

Approach #3

Above we worked on diagnosis, we are now going to switch to symptoms. My experience is that symptom-to-bacteria associations are much stronger than diagnosis to bacteria. Mileage will vary.

Oh have I mentioned that the symptom prediction from bacteria matches my symptoms almost completely? I think it’s 17 out of 20. Pretty incredible.

From a user in Europe by email on 11/11/2021

Below are his reported symptoms against predicted symptoms. It is interesting that many several predicted symptoms are autism related (which he does not have). This approach uses the bacteria that citizen science has associated to the symptoms (instead of clinical studies to the diagnosis). In theory, it will often be more sensitive for identifying the bacteria of concern.

See the video for how we do this. The final suggestions in Excel/csv format is below

Bottom Line

The intent of Microbiome Prescription site is to improve the odds of helping by working off studies on the US National Library of Medicines (at present, there are almost 6000 articles that we were able to harvest information from). We are very open on the where we get data, for example – for where we get the list of bacteria associated with a condition

From https://microbiomeprescription.com/citations/PubMedCitations?Code=LCV

And sources for how we know that something changes bacteria populations. In this case because of the high number of studies on inulin it will receive a high weight if certain bacteria are being targeted.

Example of what inulin impacts

We also try keeping faithful to the term used in the studies — apple contains large amounts of pectin, while some would just combined these to pectin (or apples), we attempt to keep the fine details. One related area that needs calling out is studies using items like  luteolin (flavonoid). If you click on these, you will go to a summary page with a link to foods containing it

We have a list of foods and amounts that contain it. It’s an extra step, but since these foods were not cited in the study, we “keep religion” and only cite what was used.

I am not licensed medically, and thus there is no clinical experience (or bias) for the suggestions. It is an uber-logical model.

With that said, this person needs to sit down with his significant other, look thru the lists and decide which options they wish to try. Being a trained pharmacist means that he can also evaluate the prescription options for risk and in some cases, try to game the system… for example: Atorvastatin … he may want to test for the conditions where it would be prescribed, if he is a little high — he may wish to use that as a “standard of care” rationale for getting a prescription — it’s an off label use (like Viagra was not intended for what it is prescribed for today).

As always, any planned action should be reviewed by their knowledgeable medical professional before starting.

EU/JRC Technical Report related

Caution for other Long COVID Patients

Before COVID, you had a unique microbiome, COVID “infection formed” it to suit its needs. These changes caused symptoms, made it easier for secondary infection and allow “alternative community of bacteria” to become established. How it changed depends on what it was like before and which variant of the virus. While the above suggestions are likely similar to what your suggestions could be, it is really important to get your own microbiome sample to work from. There will be large differences between people. With this approach, we can be single person specific for a treatment plan.

P.S. This sample was done via Biomesight, a UK based firm

Suggestions from Symptoms are Changed

A few weeks ago, I stumbled on some algorithms that had good results for predicting symptoms from bacteria. The next logical step was using the associations to get suggestions. While working on a blog post, I was getting odd results and digging into why, I discovered both logic and computational errors. Two readers had also raised questions about apparent bizarre logic. They were right — my logic was too simplistic and needed revision as well as better exposure of the logic being used.

The corrected version is up now. The main differences are:

  • Auto checking check boxes will happen less
  • Possible additions (unchecked) are marked with a 💡 
  • For Premium users, you get to see more of the gears that are turning.

To explain the issue, let us look at some details shown when in professional mode.

The old logic made suggestions to move away from the Cohort number. So for Emticicia, because we were higher than the Cohort value, we would try to raise it. This revision tries to lower it towards the 50%ile instead. The conceptual logic of moving away from the cohort was correct ignoring the sample percentile was incorrect. This implementation revision should correct this. For the other two bacteria above, we see that the cohort was high and the shift was even higher — again, moving away from the cohort is the desired, but moving higher than 90%ile is likely a poor choice, in this case you want to really move it down a lot.

The other factor is taking into account the z-score, etc. Some pages may have no automatic check. If you just click thru, you may get this message:

Let us look at some of the automatic checked

Both the person’s percentile and the Cohort are high, one was below the cohort and one was above. Because they are both high, the logic is to move them down to the middle (ignoring which side of the cohort it was on). The last one was not checked despite being Very Strong because the sample percentile was so close to the 50%ile (middle value)

A third set of examples is below, which include the weight being visible (likely will be moved to a professional feature — mainly because it can be more complicated to interpret well)

To get an automatic check the weight needs to be at least 20, for the 💡 , at least 10. For Acidobacteria, it is low but it is also a considerable distance from the cohort average. If selected, we declare a negative value and thus attempt to increase it (potentially moving it much closer to the cohort typical value — i.e. increase symptom). On the flip side, at only the 10%ile, you do not really want to decrease it more. A dilemma – excluding it is actually the best path. It has significance for the symptom forecast but has no clear action for altering the associated bacteria.

For this last one, we see Collinsella is a middle peak, and the desired direction is to increase it (negative value). Remember that these weights are used in computing the weights for suggestions.

Bottom lIne

The site is always in a state of change — from new studies being added, new samples being uploaded (and many statistics recalculated daily) and tuning and adjust algorithms — in this case readers questions lead to looking at the working data and seeing potential issues to correct (as well as displaying those numbers so people may ask questions — leading to still better algorithms).

Ketogenic diet did not help a health issue, it created one

A reader reached out for an educational review of their 16s microbiome results. I usually try to make time to do an education review once a fortnight unless I am deep in coding or analysis issues. He provided a nice very detailed back story, which is verbatim below

My problem started out approaching eight years ago when I went on a ketogenic diet.  I had a massive energy collapse and weight loss (I was not overweight at the start), and I incorrectly believed this was about insufficient calories rather than macronutrients

It turns out I have some uncommon genetics that make my ability to process fats for energy inefficient, under conditions of severe catabolic stress.   Severe catabolic stress would be conditions like starvation, high fever, etc.   Keto diet mimics starvation and is an extremely catabolic stress to the body.    It looks like this diet was a suicide diet for me, and it started a set of symptoms that are persistent even after reverting to a higher carb diet.

The original symptoms were like CFS, but I have since been able to correct most of those by going to a higher carb diet, mostly using lower-glycemic whole food carbs like lentils, fruit, and vegetables.     This took about three years to figure out and I think some infections may have exploited the period of low energy.   Those infections may include dysbiosis as well as possibly some kind of brain infection.

During the first month of the diet I had some “event” where I tried to correct the energy collapse with a higher level of exercise, and after one high-intensity running session I spent the entire night drinking a liter of water every hour.  I was desperately thirsty and was unable to quench the thirst.   

The high levels of water probably induced an electrolyte imbalance, further aggravating the underlying cause.   I should have made a trip to the emergency room, but I did not.  After that one night I started with a tinnitus that has been with me 24×7 for approaching eight years.   At first that tinnitus was like something at the brain core activating and literally consuming consciousness.   That has improved over time and in the last year feels like it could become just a sound at some point soon.   Together with the tinnitus I have some irritation of my optical nerve that gets worse when the tinnitus gets worse.   These problems are severe and constant, and I cannot survive the stress of employment with such severe stressors on my brain.   

It is worth mentioning that I was an entrepreneur for more than 10 years, working 14+ hour days.  So I am the opposite of lazy.  I had lifelong IBS-D, which is now well controlled, and most of that IBS I attribute to gluten and to dairy.  I am gluten free, and now I get my only dairy exposure from carefully prepared yogurt.  

I have neurological symptoms of brain fog (along with tinnutus) tied to food.  About 60 to 90 minutes after eating the symptoms begin.   I have multiple SIBO tests, that show an ongoing set of issues.   My first SIBO test had enormous levels of background methane.   My practitioner treated this as a colonic issue and we corrected that completely.   My next SIBO test showed completely flat-line hydrogen and methane,  a hydrogen sulfide (H2S) SIBO. I went on a heavy yogurt diet and that improved my symptoms.   I stopped the yogurt, but when I repeated a SIBO test using the Trio test, it showed totally normally hydrogen, methane, and H2S.  So I cured the SIBO.   I have organic acid markers suggesting SIFO, and since I still have neurological issues after eating, we will start to treat for SIFO soon.

In terms of microbiome testing, the main trend I perceive here is:
💥 I started with 18% butyrate producers.  I corrected this by a heavy diet of prebiotics including GOS, PHGG, and Acacia.   Butyrate producers are now close to 40%.
💥 I have near extinction levels of Bifidobacterium and Lactobacillus.  This I attribute to both antibiotics as well as not eating dairy foods for 20 years.   I simply starved these genera out of existence.   I do not tolerate Lactobacillus supplements or foods.  They induce huge levels of brain fog.   I am currently making a Bifido only yogurt that I load with prebiotics, and this has been extremely helpful to my health.   The dairy is being completely tolerated, which I suspect is because I have thriving Bifido populations eating the lactose all the way through digestion.
💥 I have low and inconsistent levels of Faecalibacterium prausnitzii which is a key species I have identified that is almost universally associated with good health.   I am addressing this by taking stewed apples as my main breakfast meal each morning.   Pectin feeds this bacteria.
💥 I have low levels of Akkermansia, suggesting possible problems with my mucosal layer.  I test with very low secretory IgA and somewhat leaky gut, so these might confirm a mucosal layer issue.   I am trying to correct this with Bacillus coagulans, which gave me some success in earlier trials.
💥 I have slight elevations of methanogens and H2S producers.   Biomesight and Thryve disagree about which species are present.    I feel my best strategy for keeping these controlled is to focus on enhancing butyrate producers and trying to re-establish my acetate producers.
💥 My Proteobacteria are usually under 4% but I seem to have a large population of Sutterella wadsworthensis, always over 1%.   This bacteria is worth calling out because we are measuring levels in the colon with 16s testing, and the research says this bacteria gets more dense as you travel up the small intestine.   So I may have a fair amount of it in my duodenum.  Since it is a gram negative bacteria, there might be some LPS issues.   I plan to treat this with the yogurt.

As far as brain infection, this is unfortunately something for which good tests do not exist.  I do show significant volume loss in my brain, confirmed by a radiologist on the MRI as well as by software that analyzes brain volume from those images.  The severity of my neurological symptoms does seem consistent with some viral load that may have been pre-existing, and that simply exploited a number of years of low energy.   There is a really interesting study out of Japan in the last few years where they treated people who had certain viruses with anti-virals and followed them for years after that.   Treating with anti-virals effectively eradicated all risk of Alzheimer’s in later years, strongly suggesting a cause and effect between resident viral infection and neurodegeneration.  This establishes some credibility for the hypothesis.   At some point I may try to find a specialist and do some test around anti-virals to see if that affects symptoms.

My first impressions

One item stood out greatly in his story, the parallelism to what is seen with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). Let me document out some of the parallelisms, I will cite just one publication for each:

He eliminated sufficient ME/CFS symptoms to likely not qualify for that diagnosis.

Ketogenic diet Literature

As usual, I go for gold-standard information from the US National Library of Medicine instead of internet rumor and snake-oil cure-alls. There are over 3800 studies. There medical cases when it is used with success (i.e. Epilepsy, Parkinson, Alzheimer’s diseases), but many of the studies have been with mice or with an apparent bias for positive results. Despite this, there was a good number of studies indicating general risks and complications. I have just cited a few studies from 2020 onwards, studies not available when this person made a regretted choice.

Clearly there are frequent downsides (beyond having DNA issues) that are not declared by advocates.

Testing Predictions

In my recent blog post, Predicted Symptoms – Performance Review, we found at least 50% of symptoms were correctly predicted using either KEGG Products or the Bacteria. This is a fresh test case sample. I forwarded the top 20 symptoms from these two predictors to the reader and he reported back. Results are below with the prediction engine reaching 60%.

SymptomZ-ScoreReader Comment
Comorbid-Mouth: Periradicular periodontitis inflammatory / chronic lesion around roots of teeth3.71I have now-well-controlled significant erosion of the gums.  It is not reversing but is hopefully not getting rapidly worse.
Comorbid: Small intestinal bacterial overgrowth (SIBO)3.35I apparently treated it and it no longer exists.
Post-exertional malaise: Worsening of symptoms after mild mental activity3.32Reading and focusing on written work quickly brings on fatigue and eye focus problems.
Age: 40-503.2150+
Autonomic Manifestations: Cortisol disorders or irregularity2.94Fasting brings on high cortisol and high fasting glucose.
DePaul University Fatigue Questionnaire : Tense muscles2.92Tense in general, not just muscles
30% hit rate
SymptomZ-ScoreReader Comment
Physical: Work-Sitting1.86☑️
Physical: Northern European1.82☑️
DePaul University Fatigue Questionnaire : Need to nap during each day1.77☑️
Neuroendocrine Manifestations: Poor gut motility1.653.5 hour transit time through small intestine
 Infection: Varicella Zoster Virus1.62I had chicken pox as a child.  Both parents had shingles.
Comorbid: Sugars cause sleep or cognitive issues1.53☑️
Physical: Long term (chronic) stress1.49☑️
DePaul University Fatigue Questionnaire : Ringing in the Ear1.4424×7 tinnitus that varies from bad to horrific
DePaul University Fatigue Questionnaire : Abnormal sensitivity to light1.41☑️
Neuroendocrine: Cold limbs (e.g. arms, legs hands)1.4Particularly in the feet, I have poor circulation
Post-exertional malaise: Worsening of symptoms after mild mental activity1.3Reading and focusing on written work quickly brings on fatigue and eye focus problems.
Immune Manifestations: Chronic Flatus / Flatulence / gas1.27☑️
60% hit rate

Some of the agreements were interesting, especially for Varicella Zoster Virus. The person does not have active, but we know because of Shingles that the virus persists. If the virus persists, then it will do some ‘taxonomy-forming’ of the gut to be friendly to it. I hope this person has gotten a Shingles Vaccinations.

My Approach

I will start by using some citizen science patterns from Microbiome Prescription. Specifically

As well as US Library of Medicine:

  • Irritable Bowel Syndrome 
  • ME/CFS with IBS 
  • Obsessive-compulsive disorder – while not diagnosed with it, it was a prediction that he agreed with

I will do a consensus report from the collection of suggestions for the 5 items cited above. I picked the largest cohort to get the best precision.

Second, I will clear the consensus report and do a naive consensus report with just the Kaltoft-Moltrup outliers and Dr. Jason Hawrelak. As with most of these educational reviews, I will often explore different paths for analysis.

Third, I will clear the consensus report and do all 15 prediction matches. I will leave it to the reader to do an uber consensus approach of everything together, including these items connected to vision:

First Pass

This is a revision based on the revised algorithms Suggestions from Symptoms are Changed.

Neurological-Audio: Tinnitus (ringing in ear) 

Neurocognitive: Unable to focus vision and/or attention 
IBD – PubMed using 6%
IBS – PubMed using 6%
ME/CFS with IBS
OBS – Pubmed 6%

We actually ended up with 100 “Safest Takes”

I downloaded the results and emailed to reader.

Second Pass

Bacteria NameAnalysis
AkkermansiaToo Low
  BacteroidesToo High
  BacteroidiaToo High
  BifidobacteriumToo Low
  Faecalibacterium prausnitziiToo Low
  MethanobrevibacterToo High
  ProteobacteriaToo High
Dr. Jason Hawrelak Targets
Bacteria NameAnalysis
  [Ruminococcus] torquesToo Low
  AggregatibacterToo High
  Blautia hydrogenotrophicaToo Low
  ChlorobaculumToo Low
Kaltoft-Moltrup Range

To confirm Kaltoft-Moltrup Ranges, I did a visual scan of his results, and there were no other extreme items. The default result was only 9 items on all lists, and they were only for Safest takes as shown below

Dropping the cut off point to 2 (from the default 3) increased the count to 18, with the items below added

It is interesting to note that we have 3 probiotics listed, none of them are Lactobacillus — the specific type that the reader reported severe issues with. Dropping the filter point to 1 (from default 3) we end up with 47 items and filtering to probiotics, we have the list below. A Lactobacillus showed up but only in combination with a prebiotic. Decreasing further, we see the following added next: bifidobacterium infantis,(probiotics), bifidobacterium longum bb536 (probiotics), bifidobacterium catenulatum,(probiotics)

Third Pass

This may seem to be a lot of work, but you can see that it may be done quickly from the YouTube video for this post. Note that we are doing only Bacteria (KEGG Products are too indirect to get suggestions). Remember that we did 12 sets of suggestions so the “Take Count” should be a matter of interest.

This is a revision based on the revised algorithms Suggestions from Symptoms are Changed. I only did the auto checked items. The second level suggestions were not checked.

Safest Takes
Safer
Likely Safe

I notices that many SAFEST items are in agreement with our first pass, i.e. Cacao, pediococcus acidilactic (probiotic), thyme (thymol, thyme oil). A few items, like clove are on the avoid list but the avoid came from a single suggestion.

KEGG Suggestions

This obtains suggestions using genes and is independent of the the above processes, all of suggestions had a very low weight of 2 (often we see numbers of 200-300), so these are likely weak suggestions, with the three best candidates below (any one of them is likely sufficient)

For supplements, only L-Cysteine appears (which was on the bottom of the list in our First Pass)

Putting it all together

In terms of probiotics, Bifidobacterium longum or bifidobacterium animalis lactis, symbioflor 2 e.coli probiotics, enterococcus faecium (probiotic),  bacillus subtilis (probiotics) and Akkermansia muciniphila (probiotic) appears to be preferred set — especially any that are not currently being taken.

I personally would advocate symbioflor 2 e.coli probiotics, (or Mutaflor) – because E.Coli probiotics appeared to make a major impact on reducing the time to recover for my own relapses/

I noticed what seems to be more than normal of polyphenols, herbs and spices. This is apparent on the third pass safest list and also second pass safest list but not on the first pass. I am inclined to ignore the first pass list for several: small number of bacteria in scope, symptoms and medical conditions were ignored.

Below you will find a YouTube of the analysis with additional commentary.

Reader feedback

“Your studies under Keto literature raise the possibility that a high fat diet may have exploded my levels of B wadsworthia during the active keto diet phase, and this alone may have promoted most of my brain fog.  That’s an interesting hypothesis I had not considered.   “

“On the various suggestions lists, do I understand that AI is not able to give us reasons for the suggestion, but rather it is just making associations between suggestions and reversal of symptoms that have been studied? ” INCORRECT, for the professional user, I detailed out the evidence as shown below

“It might help to define “Take Counts”.  Maybe you do that on the video.  What is also confusing is that on the Safer Takes list you have a “Take Net” and it is not clear how it is calculated.” The calculation something like this:

SUM( For each Bacteria (Magnitude of Shift desired + Function(Number of studies shifting in the right direction, Number of studies shifting in the wrong direction))

  • so substance with only a few (or just one) study for a bacteria will have a lower number
  • so substance with many studies for a bacteria will have a higher number
  • a bacteria that is slight off will have a lower number
  • a bacteria that is very off will have a higher number

The actual computational functions are proprietary and the results of 3 years of experiments.

“it is confusing because the same genus Bacteroides is alternately Too Low and then Too High.” There are THREE reference point the highs, the lows and the middle peaks (used for symptoms). People with a specific symptom may average at 35% of the median, so the goal is to shift you away from the 35% area. This it becomes a question of which direction? I made an “arbitrary” decision that if you are > 35% then we want to push you higher. If you are < 35%, we want to push you lower (ideally keeping you within the Kaltoft-Moltrup range of normal values). There is logic behind this “arbitrary” decision, but explaining it is complex.

Some Facebook comments

Dealing with Salicylate Issues – PubMed Review

I have been asked by a reader to do a review of the latest studies dealing with Salicylate( aka salicylic acid (SA)) Issues. The classic treatment model is to reduce or avoid salicylate foods. Some studies have reported that 2.5% Europeans may suffer from salicylate sensitivity. Aspirin is related and cited as ASA.

My starting point is a 2021 study, Effectiveness of Personalized Low Salicylate Diet in the Management of Salicylates Hypersensitive Patients: Interventional Study which cites some important points”

  • How it is cultivated impacts salicylate content.
  • The usually recognized as high foods include: legumes (e.g., lentils, beans), vegetables (e.g., cauliflowers, pickled vegetables), fruits (e.g., strawberries, plums, watermelons, raspberries), some cereals (e.g., buckwheat, oat or corn), herbs and spices
  • “It was found that the intake of food products with a low glycemic index helps to reduce symptoms in some hyperactive children” [2012]

The personalized low salicylate diet may have a positive effect on reducing self-reported symptoms of asthma, rhinosinusitis, and urticaria, although it is not effective in all patients diagnosed with hypersensitivity to ASA or NSAIDs. The low salicylates diet may be a helpful new tool to support salicylates hypersensitivity therapy, helping to mitigate the symptoms and improve patient well-being. However, further research is needed on the salicylates content of foods, and thus, some modifications of the low salicylate diet may be necessary. Further research is also needed to understand the mechanism of the effect of salicylates in food on the development of food hypersensitivity symptoms.

From Conclusion to Effectiveness of Personalized Low Salicylate Diet in the Management of Salicylates Hypersensitive Patients: Interventional Study

Understanding two forms of salicylates

Looking at amounts in foods, we need to be aware that there are two forms, free (likely to be reacted to) and bound (chemically attached to other things and much less likely to react. Additionally “On analysis, most salicylate-containing foods contain both ASA and SA, and more than one-third contain ASA alone…. ASA challenge reactivity is best regarded as a marker for intolerance to a range of natural salicylates and related dietary phenolics.” [2013]

Cooking reduces the levels by 50% often. The overall content of salicylic acid and salicylates
in food available on the European market
[2017]

IBS and Mast Cell/Histamine Issues may result

Bacteria Associated with Salicylates Sensitivity

There is nothing on PubMed – no one has done a study of the bacteria shifts seen with this condition. Fortunately, Citizen Science on Microbiome Prescription found some shifts: Comorbid: Salicylate sensitive The number of samples is low, so hopefully this will improve. The most likely bacteria are:

If you have a salicylate sensitivity and a 16s lab, you may wish to upload it and see what is suggested. Here is an example walk thru

Salicylates Lookup Table

From a variety of studies on PubMed, I extracted various measurements — always going with the highest value (for those that are sensitive, the safest).

  • Which Fruits and Vegetables Should Be Excluded from a Low-Salicylate Diet? An Analysis of Salicylic Acid in Foodstuffs in Taiwan [2018]
  • Salicylates in foods [1985]
  • The overall content of salicylic acid and salicylates in food available on the European market [2017]

On line table.

COPD and the gut microbiome

Chronic Obstructive Pulmonary Disease (COPD) is a group of diseases that make it difficult to breathe. It includes emphysema, chronic bronchitis and, in some cases, asthma [src].  A friend’s wife has it and I decided to see if there was microbiome shifts associated with it, in the naïve hope that adjusting those shifts may reduce the severity.

There are many studies on the lung microbiome [110+ at present] but modifying that microbiome does not have the usual tools available. The sole one that came to mind was Symbioflor-1, which has a study from 2001 showing a 40% drop in incidences, Influence of a bacterial immunostimulant (human Enterococcus faecalis Bacteria) on the recurrence of relapses in patients with chronic bronchitis This good Enterococcus faecalis probiotic appears to suppress the bad, drug resistanct, Enterococcus faecalis found in lung from earlier studies [1993], Symbioflor-1 is provided as a liquid that is effectively gargled with(“Keep Symbioflor 1 One minute in the mouth and gurgle in front of the swallow.“) This allows some of it to get into the throat and eventually the lung.

More Literature on Symbioflor-1 for those that are interested

A Comparative Transcriptomic Analysis of Human Placental Trophoblasts in Response to Pathogenic and Probiotic Enterococcus faecalis Interaction.
The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale (Can J Infect Dis Med Microbiol ) Vol: 2021 Issue Pages: 6655414
Pub: 2021 Epub: 2021 Jan 28 Authors Tan Q , Zeng Z , Xu F , Wei H ,
Summary Html Article Publication
Influence of Catecholamines (Epinephrine/Norepinephrine) on Biofilm Formation and Adhesion in Pathogenic and Probiotic Strains of Enterococcus faecalis.
Frontiers in microbiology (Front Microbiol ) Vol: 11 Issue Pages: 1501
Pub: 2020 Epub: 2020 Jul 24 Authors Cambronel M , Nilly F , Mesguida O , Boukerb AM , Racine PJ , Baccouri O , Borrel V , Martel J , Fécamp F , Knowlton R , Zimmermann K , Domann E , Rodrigues S , Feuilloley M , Connil N ,
Summary Html Article Publication
In silico analyses of the genomes of three new bacteriocin-producing bacteria isolated from animal`s faeces.
Archives of microbiology (Arch Microbiol ) Vol: 203 Issue 1 Pages: 205-217
Pub: 2021 Jan Epub: 2020 Aug 17 Authors Eveno M , Belguesmia Y , Bazinet L , Gancel F , Fliss I , Drider D ,
Summary Publication Publication
Probiotic Enterococcus faecalis Symbioflor 1 ameliorates pathobiont-induced miscarriage through bacterial antagonism and Th1-Th2 modulation in pregnant mice.
Applied microbiology and biotechnology (Appl Microbiol Biotechnol ) Vol: 104 Issue 12 Pages: 5493-5504
Pub: 2020 Jun Epub: 2020 Apr 20 Authors Tao Y , Huang F , Zhang Z , Tao X , Wu Q , Qiu L , Wei H ,
Summary Publication Publication
Probiotic Potential and Safety Evaluation of Enterococcus faecalis OB14 and OB15, Isolated From Traditional Tunisian Testouri Cheese and Rigouta, Using Physiological and Genomic Analysis.
Frontiers in microbiology (Front Microbiol ) Vol: 10 Issue Pages: 881
Pub: 2019 Epub: 2019 Apr 24 Authors Baccouri O , Boukerb AM , Farhat LB , Zébré A , Zimmermann K , Domann E , Cambronel M , Barreau M , Maillot O , Rincé I , Muller C , Marzouki MN , Feuilloley M , Abidi F , Connil N ,
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Draft Genome Sequences of the Probiotic Enterococcus faecalis Symbioflor 1 Clones DSM16430 and DSM16434.
Genome announcements (Genome Announc ) Vol: 4 Issue 5 Pages:
Pub: 2016 Sep 29 Epub: 2016 Sep 29 Authors Fritzenwanker M , Chakraborty A , Hain T , Zimmermann K , Domann E ,
Summary Html Article Publication
Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model.
Archives of microbiology (Arch Microbiol ) Vol: 199 Issue 2 Pages: 203-213
Pub: 2017 Mar Epub: 2016 Sep 21 Authors Neuhaus K , Lamparter MC , Zölch B , Landstorfer R , Simon S , Spanier B , Ehrmann MA , Vogel RF ,
Summary Publication Publication
In vitro comparison of the effects of probiotic, commensal and pathogenic strains on macrophage polarization.
Probiotics and antimicrobial proteins (Probiotics Antimicrob Proteins ) Vol: 6 Issue 1 Pages: 1-10
Pub: 2014 Mar Epub: Authors Christoffersen TE , Hult LT , Kuczkowska K , Moe KM , Skeie S , Lea T , Kleiveland CR ,
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Impact of actin on adhesion and translocation of Enterococcus faecalis.
Archives of microbiology (Arch Microbiol ) Vol: 196 Issue 2 Pages: 109-17
Pub: 2014 Feb Epub: 2013 Dec 21 Authors Peng Z , Krey V , Wei H , Tan Q , Vogelmann R , Ehrmann MA , Vogel RF ,
Summary Publication Publication
Complete Genome Sequence of the Probiotic Enterococcus faecalis Symbioflor 1 Clone DSM 16431.
Genome announcements (Genome Announc ) Vol: 1 Issue 1 Pages:
Pub: 2013 Jan Epub: 2013 Feb 7 Authors Fritzenwanker M , Kuenne C , Billion A , Hain T , Zimmermann K , Goesmann A , Chakraborty T , Domann E ,
Summary Html Article Publication
In vitro comparison of commensal, probiotic and pathogenic strains of Enterococcus faecalis.
The British journal of nutrition (Br J Nutr ) Vol: 108 Issue 11 Pages: 2043-53
Pub: 2012 Dec 14 Epub: 2012 Feb 21 Authors Christoffersen TE , Jensen H , Kleiveland CR , Dørum G , Jacobsen M , Lea T ,
Summary Publication Publication
Comparative genomic analysis for the presence of potential enterococcal virulence factors in the probiotic Enterococcus faecalis strain Symbioflor 1.
International journal of medical microbiology : IJMM (Int J Med Microbiol ) Vol: 297 Issue 7-8 Pages: 533-9
Pub: 2007 Nov Epub: 2007 Apr 27 Authors Domann E , Hain T , Ghai R , Billion A , Kuenne C , Zimmermann K , Chakraborty T ,
Summary Publication Publication
Functional characterization of pro-biotic pharmaceuticals by quantitative analysis of gene expression.
Arzneimittel-Forschung (Arzneimittelforschung ) Vol: 53 Issue 5 Pages: 385-91
Pub: 2003 Epub: Authors Giese T , Zimmermann K , Meuer SC ,
Summary Publication Publication
[Reduction of acute recurrence in patients with chronic recurrent hypertrophic sinusitis by treatment with a bacterial immunostimulant (Enterococcus faecalis Bacteriae of human origin].
Arzneimittel-Forschung (Arzneimittelforschung ) Vol: 52 Issue 8 Pages: 622-7
Pub: 2002 Epub: Authors Habermann W , Zimmermann K , Skarabis H , Kunze R , Rusch V ,
Summary Publication Publication
[The effect of a bacterial immunostimulant (human Enterococcus faecalis bacteria) on the occurrence of relapse in patients with].
Arzneimittel-Forschung (Arzneimittelforschung ) Vol: 51 Issue 11 Pages: 931-7
Pub: 2001 Nov Epub: Authors Habermann W , Zimmermann K , Skarabis H , Kunze R , Rusch V ,
Summary Publication Publication

Now the gut!?!

Here we have few studies.

From the last study we see VeillonellaCorynebacterium 1, Romboutsia, Aerococcus and, Megasphaera, increasing, Lachnoclostridium decreasing. Fortunately, one of our tools on Microbiome Prescription allows us to enter these shifts and then compute with AI what would counter them.

The results are shown below (remember, they are scaled so the highest value is 1 — and the value is based on not the impact, but the number of studies reporting a desirable shift)

For those not familiar with fucoidan, Fucoidan is a long chain sulfated polysaccharide found in various species of brown algae. Commercially available fucoidan is commonly extracted from the seaweed species Fucus vesiculosusCladosiphon okamuranusLaminaria japonica and Undaria pinnatifida [wikipedia]. It is available from many suppliers on Amazon, for example:

Bottom Line

When I started working on this post, I was not expecting to fine anything significant. To one’s surprise, there was an ideal study published this year using human data with different degrees of severity. There are two really strong suggestions to be discussed with your MD before starting. – most of the rest come from Artificial Intelligence on the microbiome alone and not clinical experience; yet agree with existing studies.

Remember, these suggestions are coming solely from the reported bacteria shifts with no information about the medical condition using AI. The suggestions are supported by medical studies (where there are some)

Predicted Symptoms – Performance Review

At present we have many ways to estimate symptoms from a sample. In some cases, we look at what bacteria as a group produces, in other cases just the bacteria. This post is going to look at how well different method behaves. The different approaches are fishing expeditions to see if we can find more predictive analysis tools.

Method

Today, I added the ability to see predicted symptoms against entered symptoms, as shown below


We are going to pick the samples with the most symptoms, one per user and see how well each compares. Sample B had nothing from KEGG — KEGG is works from Species (and this sample lacked any). The nu,mbers below are matches in the top 20 predicted symtp,s/

Method (Top # selected)ABCDEFGHIJKLM
Consensus (30)11161169631067144
Bacteria (20)916912111212291441112
End Products (20)1181074765973611
KEGG Enzymes (20)8085833857134
KEGG Modules (20)130151058951071059
KEGG Products (20)701076710499367
Walking a collection of samples (each from a different person), All samples had at least 80 symptoms entered.

There are challenges with the symptoms entered – namely

What we do find is that every sample had at least one method identifying at least 50% of the person’s symptoms with the highest being 80%, followed by 75%,

Second we find that predicting from bacteria or KEGG Modules had the best performance. In no case was bacteria end products nor KEGG products nor KEGG Enzymes the best. Consensus only once matched the performance of the other two and was often very bad.

Bottom Line

The above shows a strong association of the bacteria (or it’s functions) to various symptoms. It does not prove causality, but causality is my working hypothesis (at least as a catalyst or contributor to symptoms).

This means that modifying bacteria may results in reduction or elimination of many symptoms.

Microbiome Review of a SIBO etc

Background

  • A nasal swab showed large amounts of staph aureas (but no MARCoNS) and k. oxytoca.
  • A stool test from Doctor’s Data that was done prior to SIBO treatment and treatment with a rotation of probiotics and prebiotics.
  • Partially hydrolyzed guar gum (PHGG) gave me a lot of bowel mucus after a few weeks, so I stopped it.
  • Tested with NirvanaBiome

Analysis

Because of the mention of SIBO and because I had just finished refactoring Symptom to Bacteria association, I decided to start there. I do not have great expectations because published clinical studies were unable to find any significant patterns.

See Symptoms Associations post for how we are doing this

From https://www.microbiomeprescription.com/Explorer/ToSymptomsBacteriaSummary

We now see hoe well this person matches..

As expected, no significant associations

Looking at Other Explorers

  • KEGG Enzymes – nothing
  • KEGG Module:33. Very Strong: 29, Strong: 4 – this is interesting… especially since all 33 are Middle Peaks. This should be explore in time. For anyone interested in exploring,
    • beta-Oxidation, acyl-CoA synthesis
    • Biotin biosynthesis, pimeloyl-ACP/CoA => biotin
    • CAM (Crassulacean acid metabolism), light
    • Citrate cycle (TCA cycle, Krebs cycle)
    • Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate
    • CMP-KDO biosynthesis
    • Cobalamin biosynthesis, cobyrinate a,c-diamide => cobalamin
    • D-Galacturonate degradation (bacteria), D-galacturonate => pyruvate + D-glyceraldehyde 3P
    • Gluconeogenesis, oxaloacetate => fructose-6P
    • Glycogen degradation, glycogen => glucose-6P
    • Glycolysis (Embden-Meyerhof pathway), glucose => pyruvate
    • Glycolysis, core module involving three-carbon compounds
    • Histidine biosynthesis, PRPP => histidine
    • Histidine degradation, histidine => N-formiminoglutamate => glutamate
    • Inosine monophosphate biosynthesis, PRPP + glutamine => IMP
    • Isoleucine biosynthesis, pyruvate => 2-oxobutanoate
    • Leucine biosynthesis, 2-oxoisovalerate => 2-oxoisocaproate
    • Lysine biosynthesis, DAP dehydrogenase pathway, aspartate => lysine
    • NAD biosynthesis, aspartate => quinolinate => NAD
    • Pantothenate biosynthesis, valine/L-aspartate => pantothenate
    • Pentose phosphate pathway (Pentose phosphate cycle)
    • Pentose phosphate pathway, non-oxidative phase, fructose 6P => ribose 5P
    • Pentose phosphate pathway, oxidative phase, glucose 6P => ribulose 5P
    • Phosphate acetyltransferase-acetate kinase pathway, acetyl-CoA => acetate
    • Phosphatidylethanolamine (PE) biosynthesis, PA => PS => PE
    • Pimeloyl-ACP biosynthesis, BioC-BioH pathway, malonyl-ACP => pimeloyl-ACP
    • Pyrimidine ribonucleotide biosynthesis, UMP => UDP/UTP,CDP/CTP
    • Riboflavin biosynthesis, plants and bacteria, GTP => riboflavin/FMN/FAD
    • Serine biosynthesis, glycerate-3P => serine
    • Tetrahydrofolate biosynthesis, GTP => THF
    • Tryptophan biosynthesis, chorismate => tryptophan
    • Uridine monophosphate biosynthesis, glutamine (+ PRPP) => UMP
    • Valine/isoleucine biosynthesis, pyruvate => valine / 2-oxobutanoate => isoleucine
  • KEGG Product:50. Very Strong: 36, Strong: 5, Weak: 6, Very Weak: 2

Adjusting for Middle Peak

Finding middle peaks presents some challenges for suggestions. The graphics below may help explain with a middle peak is. The why is not simple, but likely a complex interaction of many things (like other bacteria)

But looking at the raw numbers will have most people seeing “nothing”

KMFunction(value) often reveal relationships that are hard to see in the original numbers

As a result of doing this post, I realized that it was possible to generate suggestions for these middle peaks. This is explained in the video below


The resulting suggestions are below. These are very blinkered suggestions that ignore extreme values.

Consensus Suggestions

See Multiple Conditions Microbiome Analysis post for more background.

I then did two common suggetions

  • Kaltoft-Moltrup Suggestions
  • Jason Hawrelak

This results in 3 items in the Consensus

KEGG Suggestions

The clear winner is Sun Wave Pharma/Bio Sun Instant probiotics – consisting of Clostridium butyricum and Bacillus mesentericus.

For supplements, we have

  • beta-alanine
  • iron
  • L-Phenylalanine
  • L-Tyrosine
  • Vitamin B-12

Medical Conditions(PubMed) had only one item that was border line item, Graves’ disease (an immune system disorder that results in the overproduction of thyroid hormones (hyperthyroidism). A simple blood test should clarify this risk.

Thanks very much – Based on a quick skim, it’s interesting that Graves comes up bc my dad has it and my TSH has been declining the last couple of times it was measured, but they didn’t check my T4, so I need to have another test soon that will look at actual hormone levels.

From person after reviewing the draft,

Putting it all together – Consensus

On my first drafts of this post, I did the manually and then realized automating it was a much better solution for me and for others.

REMINDER: The numbers are NOT the degree of impact. It is the confidence that it will help. That is, the number of bacteria shifted in the right direction, the number of studies finding the shifts and the amount of shifts we want to see.

:

There is now a better way!

I have enabled a Consensus Report on all suggestions done on a specific sample in the last 24 hours. After 24 hours, I remove the data, First, I will do the two most common suggestions:

After the 2nd one, a new choice appears on the home page sample menu as shown below

New item appears when there are two or more. You can remove them and start over as desired

I then added Comorbid: Small intestinal bacterial overgrowth (SIBO) to the mixture,

I ahd tried a fourth one, I went to Advance Suggestions and selected PubMed for SIBO

Unfortunately, there were NO MATCHES to this data source (Not a surprise)

Now, let us look at the Consensus View, it’s divided into 6 sections as shown below

NOTE: by default it is sorted by name. Double click the value column and then double click the Count column will sort it as shown below (i.e. best or worst at top of list).

Recap

This was an interesting analysis in that PubMed literature failed to find any matches, while citizen science found patterns. The patterns were often middle peaks which would not have been seen with cookbook statistical methods focused on normal distributions (bell curves)

Suggestions should be review by your medical professional. All of the items are based solely on the impact on the microbiome bacteria — often items may have adverse effect on medical conditions.

One of the confusing aspects of Microbiome Prescription is that suggestions are derived through different paths with suggestions from different paths being in partial contradiction. Each path has limited and often fuzzy data. The safest path is to merge the lists with what is in common, the next step forward (if the first step does not give you too much to do), is items that is cited in one list and not contradicted in another list.

Often what suggestions result in

Multiple Conditions Microbiome Analysis

Existing US National Library of Medicine studies are insufficient often because they report a simplistic target group is higher or lower than the controls. The latest refactor of Symptoms (via Citizen Science) actually detect what I term “middle peaks”. Middle peaks can actually be adjusted to move someone away from a symptom’s clustering of bacteria range.

.

Adjusting when our world view is a simplistic “too high” or “too low”

We are able to detect clustering of value connected to symptoms. These values may NOT be abnormal for everyone. When we compute the statistical values for those with the symptoms, we find that this group of values is abnormal.

The adjustment process is the same as for the extreme values — we want to shift the values towards the middle. If we move the values to the other side, that is actually good for improving the symptoms.

People Have Multiple Symptoms

In the past, my advice has been simple — figure out which is most important and address those. With the symptom-association refactor we can deal with multiple symptoms to a reasonable extent. I will be using actual data for a person with the following main symptoms

  • GERD
  • Crohn’s Disease
  • Mast Cell Issue

Step #1 Examines the conditions and pick your options

After logging on (Important), we go to Symptoms with Bacteria Relationships and see what we have for each of the above

Gerd does not have many samples
Crohn’s has two cases — since more samples means more accurate detection, we will use the highest one only
With Mast Cell, we see that the relationships goes down as sample size goes up — we are likely getting better resolution

Step #2 Verify that you sample is a reasonable fit

The next step is simple, for each of the above (highest samples count) we click on the link. We end up with 3 different pages, Note (in blue) that the number of bacteria is less than above? Why? because different labs report on different bacteria. We, by design, ignore any bacteria that was not reported in the sample (if you disagree, all of the needed data is available on the citizen science site for you to create your own rules and analysis with)

Step #3 Get Suggestions for Each Reasonable Fit

All of the items above were good fits. So for each we click [Create Other Samples Profile for Selected]. Strong and Very Strong are automatically selected. You can adjust the selection with the checkboxes if you wish. On the next page, just click thru (after adjusting on any desired items) on the [Show Suggestions] button

You can look at each suggestion, but we have added a Consensus Report to make combining items easier. If you return to the 16s Sample Page you will see a new button appeared indicating that 3 sets of suggestions has been recorded.

Consensus Report persists for only 24 hours or until you manually clear them

Step #4 View Consensus Report

Click on the button and you will see a drop down, select View Consensus

The Report is in 6 sections (following the pattern for Probiotic)

  • Absolute Takes — these are items with no known negative impact on any bacteria under consideration (for ALL of the suggestions sets). These are the safest items to add
  • Probable Takes — these have some known negative impact, but the likely positive impact is very good
  • Possible Takes — these have some known negative impact, but the likely positive impact is not as certain
  • Absolute Avoid– these are items with no known positive impact on any bacteria under consideration (for ALL of the suggestions sets). These are not wise choices
  • Probable Avoid– these have some known positive impact, but the likely negative impact is bad
  • Possible Avoid — these have some known positive impact, but the likely negative impact is not good

The report lists the total number of items and allows you to restrict items to a specific level of impact confidence. The usual suggestions are all scaled so the maximum impact is 1.0 The numbers here are not scaled.

Increasing the number above will reduce the size of the list. Decreasing will increase the size

Note that we can get each table sorted by clicking on the column title.

This person is already does broccoli etc regularly —
Broccoli shows up again — a duplicate record issue that I will be fixing soon
A very short list
Note that sea weed is there BUT a different one — oh the fine details!

Bottom Line and Caution

The first item is to not include symptoms that are not good fits (I am working on calculating the fit – coming soon). When I toss in other canned suggestions (Kaltoft-Moltup or Dr. Jason HawrelakRank Used:All Ranks) in, the results do not appear as good.

The second item is that it should be review by your medical professional. All of the items are based solely on the impact on the microbiome bacteria — often items may have adverse effect on medical conditions.

Again, this is done by AI and mathematical/statistical models — it is not based on clinical experience. It is not medical advice, it describes a methodology that should be discussed with your knowledgeable medical professional (where ever they are hiding).

REMEMBER: This is based on one individual microbiome and applies only to them. There are 215 bacteria for Mast Cells above. This person sample from a specific lab had 60 matches (about 30%) of which we used 56 to generate the suggestions. Another person with the same 3 items may have a totally different set of bacteria identified.

Walk thru of earlier version – minor changes

Symptoms Associations

Over the last few years, I have been trying to tease relationship out of data. I have tried a wide variety of methods and finally found one that been producing good results.

The method is conceptually straight forward:

  • Take the actual reading and apply a monotonic increasing function to it. Thus if Valuea < valueb then func(Valuea) < func(valueb)
  • With the resulting data, transform it to be a rectangular distribution for all samples
  • Hypothesis test the values from people who recorded symptoms using P=0.01 as a threshold

Once the candidate association are done then we can also test if a sample’s item satisfies the hypothesis.

This approach has some nice characteristics, because it will detect patterns that:

  • are not linear on the values
  • does not assume a normal distribution
  • does not not assume items are caused by end associations (i.e. too high or too low)
    • In some cases, we see a shift into a middle range that is statistically significant

Adjusting “Middle Peak” patterns

Both of the above above are typical beliefs that people will attempt to apply to the data.

Probability distribution function; (a) uniform distribution of the... |  Download Scientific Diagram
Comparing uniform distribution to normal distribution

Seeing the Bacteria Interactions in Your Sample

I have refactored Bacteria Interactions Why? on the site to use the data discovered via this post. The information is more accurate and more comprehensive than the prior version

I have done a quick demo, shown below, and I will add a few examples after.

Key points

  • The color of the oval indicate level of hierarchy
    • Same color to the middle one indicates that it’s independent
    • Often other colors are children or parents
  • Line thickness indicate amount of influence
  • Size of the ovals indicate percentile ranking.
    • A small oval indicates less than bacteria than normally seen
    • A large oval indicates more bacteria than normally seen

Examples

In our first example below we see many other species encourages this species If we look at it’s hierarchy on NCBI, we see a lot of bacteria that are not related by descent.

 FirmicutesNegativicutesVeillonellalesVeillonellaceaeVeillonella

Chart of Species Veillonella parvula

For the next example we see many siblings influencing it.

Bottom Line

Beyond the fun to see aspect, if there is an item of concern you may wish to see what bacteria influences it and include those in a hand-picked sample.