Special Studies: Allergies And Food Sensitivity

This is a common symptom for many people. This is reported often in samples, and thus being examined if it reaches our threshold for inclusion as defined in A new specialized selection of suggestions links. It does. We are not being specific about the type of allergy.

Sub-Series Study Populations:

We have 5 symptom annotations that this sub-series are examining, I tried different combination to see which resulted in a higher z-score to identify probable siblings (in a statistical sense)

  • New food sensitivities – 12.1 z-score
  • Medication sensitivities -8.8 z-score
    • Combined, dropped to 6.4
  • Alcohol intolerance – 8.6 z-score
    • with Medication sensitivities 9.9 z-score and less bacteria
  • Allergic Rhinitis (Hay Fever) – 8.6 z-score
    • When combined with any of the above, a major drop of z-scores
  • Allergies – 9.2 z-score
    • With new food sensitivities – 9.9 z-score
    • When combined with any of the other, a major drop of z-scores

We have broken this down into 3 sub-groups of microbiome shifts:

SymptomReferenceStudy
Allergies And Food Sensitivity113073
  • Bacteria Detected with z-score > 2.6: found 163 items, highest value was 9.9
  • Enzymes Detected with z-score > 2.6: found 364 items, highest value was 6.8
  • Compound Detected with z-score > 2.6: found No items

The bacteria that was most significant was Prevotella copri (species) which seems to occur often in these special studies.

Interesting Significant Bacteria

All bacteria found significant (except 1) had too low levels. This is a common pattern found with these studies, it is not “bad bacteria bogie man bacteria” but an absence of “upstanding citizens bacteria”. What is very striking is that with allergies, Prevotella copri is 1/10 that of the reference population, but as you move up to it’s genus (Prevotella) and family (Prevotellaceae) the ratio becomes less and less extreme. The species is where things are happening. There is one retail probiotic species listed (E.Coli – i.e. Mutaflor or Symbioflor-2) and two Bifidobacterium species which hopefully will become available one day.

” Since its discovery in 2007, the importance of the human gut bacterium Prevotella copri (P. copri) has been widely recognized with its links to diet and health status and potential as next generation probiotic” [2021]

BacteriaReference MeanStudyZ-Score
Prevotella copri (species)6619060979.9
Shuttleworthia (genus)279616.9
Prevotella (genus)73487241016.2
Tepidanaerobacter (genus)55256.2
Tepidanaerobacter syntrophicus (species)55256.2
Tepidanaerobacteraceae (family)55256.2
Prevotellaceae (family)81231331605.8
Sporolactobacillaceae (family)174605.7
Sporolactobacillus (genus)176625.6
Sporolactobacillus putidus (species)176625.6
Thermosediminibacterales (order)53185.6
Bifidobacterium catenulatum subsp. kashiwanohense (subspecies)322895.6
Bifidobacterium kashiwanohense PV20-2 (strain)321895.6
Caldilineaceae (family)91375.4
Caldilinea (genus)91375.4
Caldilineales (order)91375.4
Caldilinea tarbellica (species)91375.4
Caldilineae (class)91375.4
Lactiplantibacillus pentosus (species)119225.3
Escherichia (genus)612315495.1

Prevotella copri is called out in several studies

Interesting Enzymes

All enzymes (except 3) found significant had too low levels. Given 364 items and 99% confidence, 3 is what you would expect with False Detection Rate, so we can dismiss these one.

EnzymeReference MeanStudy MeanZ-Score
propanoyl-CoA:oxaloacetate C-propanoyltransferase (thioester-hydrolysing, 1-carboxyethyl-forming) (2.3.3.5)15133696.8
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pyruvate-lyase (succinate-forming) (4.1.3.30)14613696.5
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming] (4.2.1.79)13173856
S-methyl-5′-thioadenosine:phosphate S-methyl-5-thio-alpha-D-ribosyl-transferase (2.4.2.28)370515415.9
L-carnitinyl-CoA hydro-lyase [(E)-4-(trimethylammonio)but-2-enoyl-CoA-forming] (4.2.1.149)14723465.6
5-aminopentanoate:2-oxoglutarate aminotransferase (2.6.1.48)24077935.6
(S)-3-amino-2-methylpropanoate:2-oxoglutarate aminotransferase (2.6.1.22)24007905.6
2-dehydrotetronate isomerase (5.3.1.35)14245285.5
n/a (3.4.23.49)13333545.5
L-threonate:NAD+ 2-oxidoreductase (1.1.1.411)14305275.5
glutarate, 2-oxoglutarate:oxygen oxidoreductase ((S)-2-hydroxyglutarate-forming) (1.14.11.64)13193405.5
(R)-lactate hydro-lyase (4.2.1.130)15843485.4
L-carnitine,NAD(P)H:oxygen oxidoreductase (trimethylamine-forming) (1.14.13.239)12123335.4
UDP-N-acetyl-alpha-D-glucosamine:lipopolysaccharide N-acetyl-D-glucosaminyltransferase (2.4.1.56)10942935.3
protein-Npi-phospho-L-histidine:D-mannitol Npi-phosphotransferase (2.7.1.197)72060431585.3
acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (6,7 cis-dehydrogenating) (1.14.19.3)10883165.3
2,4,6/3,5-pentahydroxycyclohexanone 2-isomerase (5.3.99.11)89479625625.3
ATP:L-threonine O3-phosphotransferase (2.7.1.177)23814635.2
alpha-maltose-6′-phosphate 6-phosphoglucohydrolase (3.2.1.122)72165443035.1
2-acetylphloroglucinol C-acetyltransferase (2.3.1.272)179635.1
L-carnitine:CoA ligase (AMP-forming) (6.2.1.48)13875725.1
RNA-3′-phosphate:RNA ligase (cyclizing, AMP-forming) (6.5.1.4)12323715.1
D-serine ammonia-lyase (pyruvate-forming) (4.3.1.18)70096428045
(E)-4-(trimethylammonio)but-2-enoyl-CoA:L-carnitine CoA-transferase (2.8.3.21)13975635
gamma-butyrobetainyl-CoA:electron-transfer flavoprotein 2,3-oxidoreductase (1.3.8.13)14115665
3-dehydro-4-phosphotetronate carboxy-lyase (4.1.1.104)16986475

Bottom Line

There is an excellent article on different strains and how they are related to diet with discussion of KEGG Enzymes, Distinct Genetic and Functional Traits of Human Intestinal Prevotella copri Strains Are Associated with Different Habitual Diets [2021] which states ” Interestingly, Italian V and VG clustered with Western populations, suggesting that a Western plant-based diet is still not effective in establishing a P. copri strain consortium typical of a traditional agrarian diet, and supporting the existence of a geographically based distribution of different strain patterns” — which hints how a change of diet is causing an uptick in allergies.

” Prevotella copri is a bacterium naturally present .. in sauerkraut and beer.” [2020] So the German Beer Hall Diet!! 😉

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