Determining D-Lactic Acid free probiotics

For literature on D-Lactic Acidosis see these posts: [2019] [2015] [2019] or

Increased D-Lactic Acid Intestinal Bacteria in Patients with Chronic Fatigue Syndrome [2009] “This study suggests a probable link between intestinal colonization of Gram positive facultative anaerobic D-lactic acid bacteria and symptom expressions in a subgroup of patients with CFS. Given the fact that this might explain not only neurocognitive dysfunction in CFS patients but also mitochondrial dysfunction, these findings may have important clinical implications.”

There are two approaches that could be used to make this determination:

  • Growing the probiotic is a mono-culture environment and measuring it. This is the traditional approach.
  • Examining the genes and see if any contain d-lactic acid producing enzymes

I favor the second approach because there can be issues with the reliability of the first one. Some bacteria shift production of metabolites, such as d-lactic acid, based on the availability of food, supplies, other metabolites in the environment (look up quorum sensing) etc. To give a human analogy, a human blacksmith will make iron ploughs but in a war situation, they may switch to guns, spears or armor.

Using the genes approach, we know what they are capable of making (not necessarily what they are making in a specific environment). In the case of the black smith above, his shop may be incapable of casting cannons.

How do I find out data on the second approach? You could go to KEGG: Kyoto Encyclopedia of Genes and Genomes and search there. I have added that information to my Researched Retail Probiotics by Strains page.

On the far right column, you will see that some have a special link under Details.

Clicking this link will take you to the detail page where a link to KEGG data is shown

This page has four sections, two are important for this question: Produces and consume

Just enter lactic acid in the filter box to see whether it is included.

Example of a D-Lactic acid CONSUMING probiotics — likely good for the brain fogged (Lactobacillus helveticus R0052)

That is it. Remember this will only list bacteria that:

  • Have studies on PubMed with this specific strain
  • Have their gene sequence on KEGG.jp
  • Be available as a retail product somewhere in the world.

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