We have a varied history with some storms blowing us off courses. Here’s a list of the tests and prior blog posts:
- 2018: Irritable Bowel Syndrome (IBS) diagnosis
- 2021-11-18 – IBS + BioNTech COVID Vaccine -> ME/CFS? [Jan 6,2022]
- 2022-05-20 – Follow up Analysis for ME/CFS (After COVID) [Jun 16,2022]
- 2023-06-22 – Repeat IBS + Vaccine + COVID [Jul 22,2022]
- 2023-09-04 – Biomesight #4 Sample: IBS and COVID
This is one of two set of ongoing samples from readers that “have appeared to almost stalled”. The other reader sent me this note at the same time (and another sample) “I have to say, symptoms are pretty dead on accurate to what I’m experiencing like all but one box. PEM is my highest ,”
This implies strongly that the association between symptoms and bacteria shifts are strong. The microbiome is just stubborn.
Feedback
He has 49 different symptoms checked and unusually, have a friendly MD in terms of antibiotics.
I would say some small subjective improvements since last time, but no major changes. Also I increased squats from 14 to 15 and push-ups from 9 to 10 at the time of the sample. (I also increased walking distance a little, but only after the sample was taken. I probably could have increased them by more, but I wanted to start really slowly with increasing those things.)
Between the last test and this sample, Amoxicillin was the only antibiotic I did. I also did some of the top herbs and probiotics (B. infantis, Mutaflor and L. Kefiri).
Also anxiety is a bit of a problem, but only sometimes, not every day. I’m not really sure what the cause is. I think it might be connected with winter, cold, rarely seeing the sun, maybe piracetame, people around me coming to me with suggestions on what I should do, maybe noise. (Noise has been a problem for me since the beginning of CFS. I really can’t endure noise well.) But those all don’t have to be the cause, they can also just be triggers or be connected to it. (Eating might also be connected to it. It seems to me that on the days I eat bacon for breakfast and a large portion of potatoes and beef for lunch (like once a week), it is better. On the days I eat sth. made from corn flour for breakfast (like every four days), it is worse. But I cannot eat the same things multiple days in a row because I quickly develop food intolerances if I do that, so I have to eat alternating things that I tolerate.) I am just wondering whether something can be done to help with that (anxiety).
Analysis
The eubiosis index is a recent addition and I am curious on how it behaves compared to other measures. Having a magic number to indicate health is an ideological goal, my gut feeling is that there is no such magic — rather many aspects need to be examined.
Sample | Eubiosis (balanced) gut. | Unhealthy Bacteria |
2021-11-18 | 99.6 | 8 |
2022-05-20 | 48.5 | 12 |
2023-06-22 | 38.5 | 12 |
2023-09-04 | 100 | 12 |
2023-11-23 | 100 | 12 |
The traditional analysis
Criteria | 11/23/2023 | 9/4/2023 | 6/22/2023 | 5/20/2022 | 11/18/2021 |
Lab Read Quality | 5.4 | 4.7 | 7.2 | 8.1 | 5.5 |
Outside Range from GanzImmun Diagostics | 12 | 11 | 11 | 15 | 15 |
Outside Ran ge from JasonH | 7 | 9 | 9 | 6 | 6 |
Outside Range from Lab Teletest | 25 | 22 | 22 | 20 | 20 |
Outside Range from Medivere | 11 | 15 | 15 | 16 | 16 |
Outside Range from Metagenomics | 8 | 7 | 7 | 9 | 9 |
Outside Range from Microba Co-Biome | 3 | 3 | 3 | 1 | 1 |
Outside Range from MyBioma | 6 | 6 | 6 | 5 | 5 |
Outside Range from Nirvana/CosmosId | 22 | 23 | 23 | 20 | 20 |
Outside Range from Thorne (20/80%ile) | 235 | 241 | 241 | 231 | 231 |
Outside Range from XenoGene | 33 | 35 | 35 | 29 | 29 |
Outside Lab Range (+/- 1.96SD) | 2 🙂 | 21 | 7 | 8 | 6 |
Outside Box-Plot-Whiskers | 31 🙂 | 58 | 42 | 37 | 69 |
Outside Kaltoft-Moldrup | 47 🙂 | 51 | 92 | 94 | 48 |
Bacteria Reported By Lab | 508 | 568 | 582 | 674 | 527 |
Bacteria Over 90%ile | 17 🙂 | 41 | 24 | 38 | 52 |
Bacteria Under 10%ile | 83 | 50 | 147 | 177 | 36 |
Shannon Diversity Index | 1.59 | 1.272 | 1.556 | 1.853 | 1.826 |
Simpson Diversity Index | 0.047 | 0.087 | 0.09 | 0.056 | 0.038 |
Chao1 Index | 6986 | 10061 | 9016 | 14744 | 7390 |
Shannon Diversity Percentile | 43.8 | 12.7 | 40 | 81.8 | 78.2 |
Simpson Diversity Percentile | 45.3 | 77.5 | 78.6 | 54.8 | 33.6 |
Chao1 Percentile | 25 | 48.9 | 41.4 | 79.4 | 27.8 |
Pathogens | 22 | 23 | 29 | 32 | 20 |
Condition Est. Over 90%ile | 5 | 0 | 9 | 3 | 4 |
At first look, there seem to be little change. Looking at specific items, there is improvement (highlighted in red above), namely less bacteria with extremes levels. For all of the reference ranges from 3rd party labs, there is no effective change.
Going Forward
Since symptoms were also entered, we proceeded with [Just give me Suggestions Include Symptoms]. The results surprised me a little; the top items were CFS antibiotics and whole-grain barley (likely a proxy for beta glucan — see this post: Beta-Glucan: The Microbiome Fixer; ß-glucan is on the to take list). The following was sorted by Take Count descending (which means that all five sets agreed)
Personally, given the slow progress, I would be inclined to try talking a medical professional into prescribing one of these.
Let us do the usual breakdown by types, most are below 1/2 of the highest value.
- Vitamins: B1, B2, B3,B6, B7, B9, B12 are all positive; with Vitamin A being top vitamin.
- REMEMBER to insure your dosages are at least at the level used in clinical studies.
- Supplements:
- All are variations of grape products
- and the common trinity of: Hesperidin (polyphenol), diosmin,(polyphenol), Arbutin (polyphenol)
- Foods: The frequently seen mixture of whole-grain barley and walnuts which I usually translate to Barley Porridge with Walnuts every day.
- Probiotics
- bifidobacterium animalis lactis (probiotics)
- bifidobacterium longum bb536 (probiotics)
- bifidobacterium breve (probiotic)
- and one that is only available in Italy: lactobacillus kefiri (NOT KEFIR)
- For sugars: sucralose(a.k.a. Splenda) and aspartame (sweetner), all natural sugars are negative.
Further analysis
I did a second run, but excluded symptoms, there was no significant shifts. The top items were the same but other antibiotics shifted position.
From the early draft, this reader responded with more information:
- He has started nicotine patches which has a significant positive score of 113
I used the ability to do a double sort (Descending Take Count, Descending Priority) to get a double conservative list.
Tudca is Tauroursodeoxycholic Acid and available as a supplement. Based on this clinical study: Safety, Efficacy and Pharmacokinetics of Doxycycline Plus Tauroursodeoxycholic Acid in Transthyretin Amyloidosis, I would suggest that it be taken concurrently with the metronidazole. There are 34 clinical studies using it. It is interesting that metformin (a diabetes drug) is suggested at the same time we see avoid all natural sugars in our suggestions – there is an interesting symmetry here. Teicoplanin is also interesting and very suitable (literature here). Literature for Ceforanide is here.
My prescription model to be discussed with the MD would be:
- Week 1:A course of metronidazole with Tudca
- Week 2-4: metformin (possibly with Tudca)
- Week 5: Teicoplanin with Tudca
- Week 6-8: metformin (possibly with Tudca)
- At end of week 8 do another sample
- Week 9: Ceforanide with Tudca
- Week 10- new report: metformin (possibly with Tudca)
Barley porridge and similar alternating with beta-glucan supplements (since food sensitivities appear to develop quickly). Perhaps consider a diabetes-like diet (to keep the natural sugars low).
For Tudca, we read about some possible adverse effects in this paper Pharmacokinetics, Safety, and Tolerability of Orally Administered Ursodeoxycholic Acid in Patients With Parkinson’s Disease—A Pilot Study [2021] namely ” gastrointestinal discomfort, rated by subjects as mild to moderate“. It has a half-life of 2.1 ± 0.71 hr, so taking 4 staggered 500mg dosages per day should be considered.
Speculation: monitoring blood glucose levels (like what diabetics do) may be a waste or time OR may provide insight. I have a smart watch that monitors blood glucose ($30 from Banggoods to Temu). There are also more professional monitors.
Question: I ate cornflour for breakfast, which I wrote about in my first mail. On those days anxiety is worse
- This is often cited on influencer’s health pages, we have some potential researched supporting it:
- TRPM2 may be involved in high fructose corn syrup-induced anxiety-like behavior in adult male rats [2023]
- High Fructose Corn Syrup-Moderate Fat Diet Potentiates Anxio-Depressive Behavior and Alters Ventral Striatal Neuronal Signaling.[2021]
- However, blue maize may reverse it:
- Effect of consumption of blue maize tortilla on anxiety-like behaviour, learning, memory and hippocampal BDNF expression in a chronic stress model in rats. [2023] “The consumption of anthocyanin extract or tortilla made with blue corn decreased anxiety-like behaviours”
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